Pre-storage incubation for 6 or 8 h elevated significantly the grade A chicks and reduced the grade B chicks in comparison with non-heated controls. At 28 and 50 weeks of age, pre-storage incubation and its interaction with storage period influenced significantly the apparent fertility, hatchability of set eggs and hatchability of fertile eggs and this improvement in hatchability is attributed to the reduction in embryonic mortality (early, intermediate and late). In each breeder flock age, eggs were distributed in a completely randomized experimental design in a 2×4 factorial arrangement, with two storage periods (4 or 14 days) and four pre-storage incubation durations (0, 4, 6 or 8 h at 37.5☌). Half of the eggs were collected from young breeder hens (28 weeks old) and the other half from old breeder hens (50 weeks old). A total of 8000 fertile eggs were obtained from two different ages of chicken breeder hens (Egyptian local cross, Inshas). The objective of the present study was to investigate the effects of pre-storage incubation and storage time on hatchability characteristics, chick quality and serum thyroid hormones, antioxidative properties and immunoglobulin Y (IgY) concentrations of newly hatched chicks at two breeder flock ages. ![]() Pre-storage incubation has been a suggested method to reduce the negative effects of long-term storage times by enhancing the developmental stage of the embryo and probably reducing the embryonic stress. Egg storage longer than 7 days is associated with negative effects on hatchability traits.
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